In this survey we examined 87 domestic animal stool samples in order to detect the possible presence of microsporidia in animals in close contact with humans in Galicia (NW, Spain). The detection of Enterocytozoon bieneusi
spores was confirmed in faecal samples from two dogs and one goat by polymerase chain reaction. None of the positive samples for microsporidia in the staining method were amplified with species-specific primers for Encephalitozoon intestinalis
, E. hellem
and E. cuniculi
. Four rabbits faecal samples reacted with anti-E. cuniculi
serum. Our results could indicate the importance of domestic animals as zoonotic reservoirs of microsporidial human infections.