Candida albicans check for this species in other resources - molecular typing - random amplified polymorphic DNA - population structure - Nicaragua"/>
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Memórias do Instituto Oswaldo Cruz
Fundação Oswaldo Cruz, Fiocruz
ISSN: 1678-8060
EISSN: 1678-8060
Vol. 97, No. 7, 2002, pp. 985-989
Bioline Code: oc02222
Full paper language: English
Document type: Research Article
Document available free of charge

Memórias do Instituto Oswaldo Cruz, Vol. 97, No. 7, 2002, pp. 985-989

 en First Characterization of Candida albicans check for this species in other resources by Random Amplified Polymorphic DNA Method in Nicaragua and Comparison of the Diagnosis Methods for Vaginal Candidiasis in Nicaraguan Women
Martha Darce Bello; Alcides Gonzalez; Christian Barnabé & Georges Larrouy

Abstract

A total of 106 women with vaginitis in Nicaragua were studied. The positive rate for the identification of Candida check for this species in other resources species was 41% (44 positive cultures out of 106 women with vaginitis). The sensitivity of microscopic examination of wet mount with the potassium hydroxide (KOH) was 61% and 70% with Gram's stain when using the culture of vaginal fluid as gold standard for diagnosis of candidiasis. Among the 44 positives cultures, isolated species of yeast from vaginal swabs were Candida (59%), C. tropicalis (23%), C. glabrata (14%) and C. krusei (4%). This study reports the first characterization of 26 Candida stocks from Nicaragua by the random amplified polymorphic DNA method. The genetic analysis in this small Candida population showed the existence of linkage disequilibrium, which is consistent with the hypothesis that Candida undergoes a clonal propagation.

Keywords
vulvovaginal candidiasis - diagnosis - Candida albicans check for this species in other resources - molecular typing - random amplified polymorphic DNA - population structure - Nicaragua

 
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