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Memórias do Instituto Oswaldo Cruz
Fundação Oswaldo Cruz, Fiocruz
ISSN: 1678-8060
EISSN: 1678-8060
Vol. 97, No. 7, 2002, pp. 991-995
Bioline Code: oc02223
Full paper language: English
Document type: Research Article
Document available free of charge

Memórias do Instituto Oswaldo Cruz, Vol. 97, No. 7, 2002, pp. 991-995

 en Complete Nucleotide Sequence Analysis of a Brazilian Dengue Virus Type 2 Strain
Flávia Barreto dos Santos; Marize Pereira Miagostovich; Rita Maria Ribeiro Nogueira; Dianna Edgil; Hermann Gonçalves Schatzmayr; Lee W Riley & Eva Harris

Abstract

In the last decade, dengue fever (DF) in Brazil has been recognized as an important public health problem, and an increasing number of dengue haemorrhagic fever (DHF) cases have been reported since the introduction of dengue virus type 2 (DEN-2) into the country in 1990. In order to analyze the complete genome sequence of a DEN-2 Brazilian strain (BR64022/98), we designed primers to amplify contiguous segments of approximately 500 base pairs across the entire sequence of the viral genome. Twenty fragments amplified by reverse transcriptase-PCR were cloned, and the complete nucleotide and the deduced amino acid sequences were determined. This constitutes the first complete genetic characterization of a DEN-2 strain from Brazil. All amino acid changes differentiating strains related to the Asian/American-Asian genotype were observed in BR64022/98, indicating the Asiatic origin of the strain.

Keywords
dengue virus type 2 - complete genome - Brazil

 
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