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Memórias do Instituto Oswaldo Cruz
Fundação Oswaldo Cruz, Fiocruz
ISSN: 1678-8060
EISSN: 1678-8060
Vol. 97, No. 7, 2002, pp. 1009-1013
Bioline Code: oc02226
Full paper language: English
Document type: Research Article
Document available free of charge

Memórias do Instituto Oswaldo Cruz, Vol. 97, No. 7, 2002, pp. 1009-1013

 en Detection of Antibodies to the 97 kDa Component of Toxoplasma gondii check for this species in other resources in Samples of Human Serum
Alessandra Carla de Almeida Ribeiro; Maria Aparecida de Souza & José Roberto Mineo

Abstract

This study was carried out to investigate the immune response against 97 kDa (p97) molecular marker of Toxoplasma gondii check for this species in other resources that has been characterized as a cytosolic protein and a component of the excreted-secreted antigens from this parasite. A total of 60 serum samples from patients were analyzed by enzime-linked immunosorbent assay and Western blot for toxoplasmosis. These samples were organized in three groups, based on clinical symptoms and results of serological tests. Group I: 20 samples reactive to IgG and IgM (acute phase); group II: 20 non-reactive samples (control group); and group III: 20 samples reactive only to IgG (chronic phase). Western blot was performed with total antigenic extracts or with excreted and secreted antigen from T. gondii to identify the fraction correspondent to p97. It was observed that this cytosolic component from T. gondii stimulates the immunologic system to produce both IgM and IgG antibodies in the beginning of the acute infection and IgG throughout the chronic stage of the asymptomatic toxoplasmosis.

Keywords
Toxoplasma - enzime-linked immunosorbent assay - Western blot - p97

 
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