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Memórias do Instituto Oswaldo Cruz
Fundação Oswaldo Cruz, Fiocruz
ISSN: 1678-8060
EISSN: 1678-8060
Vol. 97, No. 7, 2002, pp. 1015-1018
Bioline Code: oc02227
Full paper language: English
Document type: Research Article
Document available free of charge

Memórias do Instituto Oswaldo Cruz, Vol. 97, No. 7, 2002, pp. 1015-1018

 en Evaluation of the Direct Agglutination Test and the rK39 Dipstick Test for the Sero-diagnosis of Visceral Leishmaniasis
Henk DFH Schallig; Marilene Canto-Cavalheiro & Eduardo S da Silva

Abstract

The direct agglutination test (DAT) based on a freeze-dried antigen and the rK39 dipstick test were evaluated for the sero-diagnosis of visceral leishmaniasis (VL). The sensitivity and specificity of both tests were determined using sera from confirmed VL patients (n = 21), healthy controls (n = 19) and from patients with other confirmed infectious diseases (n = 42). The DAT had a sensitivity and a specificity of 100%. The rK39 had a sensitivity of 85.7% and a specificity of 82%. Both tests were also used to screen blood samples of confirmed VL patients (n = 15) and serum samples of VL suspects (n = 61). The DAT found all blood samples of confirmed VL patients positive and tested 98.4% of the serum samples of the VL suspects positive. In contrast, rK39 detected in 9/15 blood samples (60%) antibodies against Leishmania chagasi check for this species in other resources and found 85.3% of the serum samples of the suspected patients positive. Although the rK39 dipstick is more rapid and user friendlier than the DAT, the latter has a superior sensitivity and specificity. Furthermore, the reagents used for DAT do not require cold storage, whereas the buffer of the rK39 must be stored at 4°C. Therefore, the DAT is the most suitable test for the sero-diagnosis of VL under field conditions.

Keywords
visceral leishmaniasis - direct agglutination test - rK39 dipstick test - Leishmania chagasi check for this species in other resources - diagnosis - human - Brazil

 
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