Leishmania check for this species in other resources (L.) major - rhesus macaques ( Macaca mulatta check for this species in other resources ) - vaccination - attenuated and killed promastigotes - bacillus Calmete-Guerin (BCG)"/>
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Memórias do Instituto Oswaldo Cruz
Fundação Oswaldo Cruz, Fiocruz
ISSN: 1678-8060
EISSN: 1678-8060
Vol. 97, No. 7, 2002, pp. 1041-1048
Bioline Code: oc02231
Full paper language: English
Document type: Research Article
Document available free of charge

Memórias do Instituto Oswaldo Cruz, Vol. 97, No. 7, 2002, pp. 1041-1048

 en Study of the Safety, Immunogenicity and Efficacy of Attenuated and Killed Leishmania check for this species in other resources (Leishmania) major Vaccines in a Rhesus Monkey ( Macaca mulatta check for this species in other resources ) Model of the Human Disease
VF Amaral; A Teva; MP Oliveira-Neto; AJ Silva; MS Pereira; E Cupolillo; R Porrozzi; SG Coutinho; C Pirmez; SM Beverley & G Grimaldi Jr

Abstract

We have compared the efficacy of two Leishmania check for this species in other resources (Leishmania) major vaccines, one genetically attenuated (DHFR-TS deficient organisms), the other inactivated [autoclaved promastigotes (ALM) with bacillus Calmete-Guérin (BCG)], in protecting rhesus macaques ( Macaca mulatta check for this species in other resources ) against infection with virulent L. (L.) major. Positive antigen-specific recall proliferative response was observed in vaccinees (79% in attenuated parasite-vaccinated monkeys, versus 75% in ALM-plus-BCG-vaccinated animals), although none of these animals exhibited either augmented in vitro gamma interferon (IFN-γ) production or positive delayed-type hypersensitivity (DTH) response to the leishmanin skin test prior to the challenge. Following challenge, there were significant differences in blastogenic responses (p < 0.05) between attenuated-vaccinated monkeys and naïve controls. In both vaccinated groups very low levels of antibody were found before challenge, which increased after infective challenge. Protective immunity did not follow vaccination, in that monkeys exhibited skin lesion at the site of challenge in all the groups. The most striking result was the lack of pathogenicity of the attenuated parasite, which persisted in infected animals for up to three months, but were incapable of causing disease under the conditions employed. We concluded that both vaccine protocols used in this study are safe in primates, but require further improvement for vaccine application.

Keywords
Leishmania check for this species in other resources (L.) major - rhesus macaques ( Macaca mulatta check for this species in other resources ) - vaccination - attenuated and killed promastigotes - bacillus Calmete-Guerin (BCG)

 
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