Trypanosoma cruzi check for this species in other resources - clone CL B5 - biological characterization"/>
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Memórias do Instituto Oswaldo Cruz
Fundação Oswaldo Cruz, Fiocruz
ISSN: 1678-8060
EISSN: 1678-8060
Vol. 97, No. 8, 2002, pp. 1101-1105
Bioline Code: oc02241
Full paper language: English
Document type: Research Article
Document available free of charge

Memórias do Instituto Oswaldo Cruz, Vol. 97, No. 8, 2002, pp. 1101-1105

 en Biological Characterization of a β-Galactosidase Expressing Clone of Trypanosoma cruzi check for this species in other resources CL Strain
Ana Le-Senne; Susana Muelas-Serrano; Carlos Fernández-Portillo; José Antonio Escario & Alicia Gómez-Barrio

Abstract

Clone CL B5 of Trypanosoma cruzi check for this species in other resources is a β-galactosidase expressing organism that was genetically transfected to be used for in vitro pharmacological screening. Biological parameters were determined, evaluating growth kinetics of epimastigotes, metacyclogenesis, infectivity to mammalian cell lines, parasitemia kinetics in mice and sensibility to nifurtimox and benznidazole. Differences in relation to other strains and CL parental strain were found, the most important being the incapability to produce death to mice in spite of the high inoculum used. However, it possesses the required features to be used for in vitro drug screening. Data obtained demonstrate that heterogeneity of T. cruzi appears even among clones of the same strain, and that these differences found do not prevent the use of clone CL B5 for the purpose that was engineered.

Keywords
Trypanosoma cruzi check for this species in other resources - clone CL B5 - biological characterization

 
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