Alomia myriadenia check for this species in other resources - Gaylussacia brasiliensis check for this species in other resources - Himatanthus obovatus check for this species in other resources - lymphocyte proliferation - immunomodulators"/>
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Memórias do Instituto Oswaldo Cruz
Fundação Oswaldo Cruz, Fiocruz
ISSN: 1678-8060
EISSN: 1678-8060
Vol. 97, No. 8, 2002, pp. 1207-1212
Bioline Code: oc02259
Full paper language: English
Document type: Research Article
Document available free of charge

Memórias do Instituto Oswaldo Cruz, Vol. 97, No. 8, 2002, pp. 1207-1212

 en Screening and Fractionation of Plant Extracts with Antiproliferative Activity on Human Peripheral Blood Mononuclear Cells
Elaine M Souza-Fagundes; Ana BR Queiroz; Olindo Assis Martins Filho; Giovanni Gazzinelli; Rodrigo Corrêa-Oliveira; Tânia MA Alves & Carlos L Zani

Abstract

Three hundred and thirteen extracts from 136 Brazilian plant species belonging to 36 families were tested for their suppressive activity on phytohemaglutinin (PHA) stimulated proliferation of human peripheral blood mononuclear cells (PBMC). The proliferation was evaluated by the amount of [3H]-thymidine incorporated by the cells. Twenty extracts inhibited or strongly reduced the proliferation in a dose-dependent manner at doses between 10 and 100 µg/ml. Three of these extracts appeared to be non-toxic to lymphocytes, according to the trypan blue permeability assay and visual inspection using optical microscopy. Bioassay-guided fractionation of Alomia myriadenia check for this species in other resources extract showed that myriadenolide, a labdane diterpene known to occur in this species, could account for the observed activity of the crude extract. Using a similar protocol, an active fraction of the extract from Gaylussacia brasiliensis check for this species in other resources was obtained. Analysis of the 1H and 13C NMR spectra of this fraction indicates the presence of an acetylated triterpene whose characterization is underway. The extract of Himatanthus obovatus check for this species in other resources is currently under investigation.

Keywords
plant extracts - Alomia myriadenia check for this species in other resources - Gaylussacia brasiliensis check for this species in other resources - Himatanthus obovatus check for this species in other resources - lymphocyte proliferation - immunomodulators

 
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