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Memórias do Instituto Oswaldo Cruz
Fundação Oswaldo Cruz, Fiocruz
ISSN: 1678-8060
EISSN: 1678-8060
Vol. 98, No. s1, 2003, pp. 59-62
Bioline Code: oc03011
Full paper language: English
Document type: Research Article
Document available free of charge

Memórias do Instituto Oswaldo Cruz, Vol. 98, No. s1, 2003, pp. 59-62

 en Random Amplified Polymorphic DNA Analysis of DNA Extracted from Trichuris trichiura check for this species in other resources (Linnaeus, 1771) Eggs and its Prospective Application to Paleoparasitological Studies
Elaine Machado Martinez; Jorge Antonio Santos Correia; Erika Verissimo Villela; Antonio Nascimento Duarte; Luiz Fernando Ferreira & Alexandre Ribeiro Bello

Abstract

Random amplified polymorphic DNA analysis was applied to DNAs extracted from Trichuris trichiura check for this species in other resources eggs recovered from human fecal samples. Four out of 6 primers tested displayed 18 distinct and well defined polymorphic patterns, ranging from 650 to 3200 base pairs. These results, upon retrieval and DNA sequencing of some of these bands from agarose gels, might help in establishing T. trichiura specific genetic markers, not available yet, and an important step to design primers to be used in molecular diagnosis approaches.

Keywords
random amplified polymorphic DNA analysis - Trichuris trichiura check for this species in other resources - paleoparasitology

 
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