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Memórias do Instituto Oswaldo Cruz
Fundação Oswaldo Cruz, Fiocruz
ISSN: 1678-8060
EISSN: 1678-8060
Vol. 98, No. s1, 2003, pp. 63-65
Bioline Code: oc03012
Full paper language: English
Document type: Research Article
Document available free of charge

Memórias do Instituto Oswaldo Cruz, Vol. 98, No. s1, 2003, pp. 63-65

 en Analysis of Ancient DNA from Coprolites: a Perspective with Random Amplified Polymorphic DNA-Polymerase Chain Reaction Approach
Alena M Iñiguez; Adauto Araújo; Luiz Fernando Ferreira & Ana Carolina P Vicente

Abstract

The aim of this work was to determine approaches that would improve the quality of ancient DNA (aDNA) present in coprolites to enhance the possibility of success in retrieving specific sequence targets. We worked with coprolites from South American archaeological sites in Brazil and Chile dating up to 7,000 years ago. Using established protocols for aDNA extraction we obtained samples showing high degradation as usually happens with this kind of material. The reconstructive polymerization pretreatment was essential to overcome the DNA degradation and the serial dilutions helped with to prevent polymerase chain reaction (PCR) inhibitors. Moreover, the random amplified polymorphic DNA-PCR has been shown to be a reliable technique for further experiments to recover specific aDNA sequences.

Keywords
molecular paleoparasitology - coprolites - random amplified polymorphic DNA - ancient DNA - polymerase chain reaction

 
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