Memórias do Instituto Oswaldo Cruz
Fundação Oswaldo Cruz, Fiocruz
Vol. 98, No. s1, 2003, pp. 63-65
Bioline Code: oc03012
Full paper language: English
Document type: Research Article
Document available free of charge
Memórias do Instituto Oswaldo Cruz, Vol. 98, No. s1, 2003, pp. 63-65
© Copyright 2003 - Instituto Oswaldo Cruz - Fiocruz
Analysis of Ancient DNA from Coprolites: a Perspective with Random Amplified Polymorphic DNA-Polymerase Chain Reaction Approach|
Alena M Iñiguez; Adauto Araújo; Luiz Fernando Ferreira & Ana Carolina P Vicente
The aim of this work was to determine approaches that would improve the quality
of ancient DNA (aDNA) present in coprolites to enhance the possibility of success
in retrieving specific sequence targets. We worked with coprolites from South
American archaeological sites in Brazil and Chile dating up to 7,000 years ago.
Using established protocols for aDNA extraction we obtained samples showing high
degradation as usually happens with this kind of material. The reconstructive
polymerization pretreatment was essential to overcome the DNA degradation and
the serial dilutions helped with to prevent polymerase chain reaction (PCR) inhibitors.
Moreover, the random amplified polymorphic DNA-PCR has been shown to be a reliable
technique for further experiments to recover specific aDNA sequences.
molecular paleoparasitology - coprolites - random amplified polymorphic DNA - ancient DNA - polymerase chain reaction
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