A molecular paleoparasitological diagnostic
approach was developed for Enterobius vermicularis
DNA was extracted from 27 coprolites from archaeological sites in Chile and USA.
Enzymatic amplification of human mtDNA sequences confirmed the human origin. We
designed primers specific to the E. vermicularis
5S ribosomal RNA spacer
region and they allowed reproducible polymerase chain reaction identification
of ancient material. We suggested that the paleoparasitological microscopic identification
could accompany molecular diagnosis, which also opens the possibility of sequence
analysis to understand parasite-host evolution.