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Memórias do Instituto Oswaldo Cruz
Fundação Oswaldo Cruz, Fiocruz
ISSN: 1678-8060
EISSN: 1678-8060
Vol. 98, No. s1, 2003, pp. 67-69
Bioline Code: oc03013
Full paper language: English
Document type: Research Article
Document available free of charge

Memórias do Instituto Oswaldo Cruz, Vol. 98, No. s1, 2003, pp. 67-69

 en Enterobius vermicularis check for this species in other resources : Ancient DNA from North and South American Human Coprolites
Alena M Iñiguez; Karl J Reinhard; Adauto Araújo; Luiz Fernando Ferreira & Ana Carolina P Vicente

Abstract

A molecular paleoparasitological diagnostic approach was developed for Enterobius vermicularis check for this species in other resources . Ancient DNA was extracted from 27 coprolites from archaeological sites in Chile and USA. Enzymatic amplification of human mtDNA sequences confirmed the human origin. We designed primers specific to the E. vermicularis 5S ribosomal RNA spacer region and they allowed reproducible polymerase chain reaction identification of ancient material. We suggested that the paleoparasitological microscopic identification could accompany molecular diagnosis, which also opens the possibility of sequence analysis to understand parasite-host evolution.

Keywords
ancient DNA - Enterobius vermicularis check for this species in other resources - coprolites

 
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