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Memórias do Instituto Oswaldo Cruz
Fundação Oswaldo Cruz, Fiocruz
ISSN: 1678-8060
EISSN: 1678-8060
Vol. 98, No. 2, 2003, pp. 255-264
Bioline Code: oc03064
Full paper language: English
Document type: Research Article
Document available free of charge

Memórias do Instituto Oswaldo Cruz, Vol. 98, No. 2, 2003, pp. 255-264

 en Human Mixed Infections of Leishmania check for this species in other resources spp. and Leishmania-Trypanosoma cruzi in a Sub Andean Bolivian Area: Identification by Polymerase Chain Reaction/hybridization and Isoenzyme
B Bastrenta; N Mita; R Buitrago; F Vargas; M Flores; M Machane; N Yacsik; M Torrez; F Le Pont & F Brenière

Abstract

Parasites belonging to Leishmania braziliensis check for this species in other resources , Leishmania donovani check for this species in other resources , Leishmania mexicana check for this species in other resources complexes and Trypanosoma cruzi check for this species in other resources (clones 20 and 39) were searched in blood, lesions and strains collected from 28 patients with active cutaneous leishmaniasis and one patient with visceral leishmaniasis. PCR-hybridization with specific probes of Leishmania check for this species in other resources complexes ( L. braziliensis , L. donovani and L. mexicana ) and T. cruzi clones was applied to the different DNA samples. Over 29 patients, 8 (27.6%) presented a mixed infection Leishmania complex species, 17 (58.6%) a mixed infection Leishmania-T. cruzi , and 4 (13.8%) a multi Leishmania-T. cruzi infection. Several patients were infected by the two Bolivian major clones 20 and 39 of T. cruzi (44.8%). The L. braziliensis complex was more frequently detected in lesions than in blood and a reverse result was observed for L. mexicana complex. The polymerase chain reaction-hybridization design offers new arguments supporting the idea of an underestimated rate of visceral leishmanisis in Bolivia. Parasites were isolated by culture from the blood of two patients and lesions of 10 patients. The UPGMA (unweighted pair-group method with arithmetic averages) dendrogram computed from Jaccard's distances obtained from 11 isoenzyme loci data confirmed the presence of the three Leishmania complexes and undoubtedly identified human infections by L. (V.) braziliensis , L. (L.) chagasi and L. (L.) mexicana species. Additional evidence of parasite mixtures was visualized through mixed isoenzyme profiles, L. (V.) braziliensis-L. (L.) mexicana and Leishmania spp.- T. cruzi . The epidemiological profile in the studied area appeared more complex than currently known. This is the first report of parasitological evidence of Bolivian patients with trypanosomatidae multi infections and consequences on the diseases' control and patient treatments are discussed.

Keywords
leishmaniasis - Chagas disease - mixed infection - isoenzyme characterization - polymerase chain reaction

 
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