We describe a method for culturing over 90% pure bovine macrophages from peripheral
blood mononuclear cells separated with Nycoprep. The cells were cultured for
12 days and then stained with esterase and with anti CD14 to test for purity.
The method is reproducible and ensures an adequate number of cells for immunological
research. Additionally, we report the unexpected finding of
trypomastigotes in our macrophage cultures from bovines belonging to a geographic
area from which no bovine trypanosomes had been reported before.