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Memórias do Instituto Oswaldo Cruz
Fundação Oswaldo Cruz, Fiocruz
ISSN: 1678-8060
EISSN: 1678-8060
Vol. 98, No. 2, 2003, pp. 269-271
Bioline Code: oc03066
Full paper language: English
Document type: Research Article
Document available free of charge

Memórias do Instituto Oswaldo Cruz, Vol. 98, No. 2, 2003, pp. 269-271

 en SHORT COMMUNICATION - Standardization of Bovine Macrophage Monolayers and Isolation and Culture of Trypanosomes
Omar A Saldarriaga; Jaime I Velásquez; Jorge E Ossa & Maria T Rugeles


We describe a method for culturing over 90% pure bovine macrophages from peripheral blood mononuclear cells separated with Nycoprep. The cells were cultured for 12 days and then stained with esterase and with anti CD14 to test for purity. The method is reproducible and ensures an adequate number of cells for immunological research. Additionally, we report the unexpected finding of Trypanosoma check for this species in other resources trypomastigotes in our macrophage cultures from bovines belonging to a geographic area from which no bovine trypanosomes had been reported before.

macrophages - bovine - trypanosomes

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