Chaptalia nutans check for this species in other resources - Asteraceae - traditional medicine - antibacterial activity - 7-O- β -D-glucopyranosyl-nutanocoumarin"/>
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Memórias do Instituto Oswaldo Cruz
Fundação Oswaldo Cruz, Fiocruz
ISSN: 1678-8060
EISSN: 1678-8060
Vol. 98, No. 2, 2003, pp. 283-286
Bioline Code: oc03069
Full paper language: English
Document type: Research Article
Document available free of charge

Memórias do Instituto Oswaldo Cruz, Vol. 98, No. 2, 2003, pp. 283-286

 en In Vitro Antibacterial Activity of a 7-O- β -D-glucopyranosyl-nutanocoumarin from Chaptalia nutans check for this species in other resources (Asteraceae)
Maria da Conceição Torrado Truiti; Maria Helena Sarragiotto; Benício Alves de Abreu Filho; Celso Vataru Nakamura & Benedito Prado Dias Filho

Abstract

Ethanolic crude extracts from the roots of Chaptalia nutans check for this species in other resources , traditionally used in Brazilian folk medicine, were screened against Staphylococcus aureus check for this species in other resources , Escherichia coli check for this species in other resources , and Pseudomonas aeruginosa check for this species in other resources by using the disk diffusion test technique. S. aureus with 14 mm inhibition zone was considered susceptible. E. coli and P. aeruginosa without such a zone were considered resistant. As a result of this finding, the ethanolic crude extract was fractionated on silica gel column chromatography into five fractions. The ethyl acetate fraction was active against S. aureus and Bacillus subtilis check for this species in other resources . Further column chromatography separation of the ethyl acetate fraction afforded 30 fractions, which were assayed against S. aureus . Fractions 16 and 17 showed inhibition zones with S. aureus , indicating the presence of active compounds, and were subjected to purification by repeated preparative thin layer chromatography. The pure compound 7-O- β -D-glucopyranosyl-nutanocoumarin inhibited B. subtilis and S. aureus at concentrations of 62.5 µ g/ml and 125 µ g/ml, respectively. The antibacterial property of C. nutans appears to have justified its use for the treatment of wounds, which are contaminated through bacterial infections.

Keywords
Chaptalia nutans check for this species in other resources - Asteraceae - traditional medicine - antibacterial activity - 7-O- β -D-glucopyranosyl-nutanocoumarin

 
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