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Memórias do Instituto Oswaldo Cruz
Fundação Oswaldo Cruz, Fiocruz
ISSN: 1678-8060
EISSN: 1678-8060
Vol. 98, No. 2, 2003, pp. 287-290
Bioline Code: oc03070
Full paper language: English
Document type: Research Article
Document available free of charge

Memórias do Instituto Oswaldo Cruz, Vol. 98, No. 2, 2003, pp. 287-290

 en SHORT COMMUNICATION - Preparation of Sand Fly (Diptera: Psychodidae: Phlebotominae) Specimens for Histological Studies
Beatriz Gomes Brazil; José Lino-Neto & Reginaldo Peçanha Brazil

Abstract

Phlebotominae sand fly specimens were prepared for histological and physiological studies. Different fixatives were tested on sectioned and whole bodied adult females in order to obtain good fixation and provide satisfactory penetration of the embedding media. All fixed specimens were infiltrated (up to seven days under 5ºC) and embedded in hydroxyethyl metacrylate. Two-three µm sections were stained, mounted in Canada balsam and observed by light microscopy. Best results were achieved when whole bodied insects were double fixed in Bouin's and Carnoy's fluids (4 h/2 h) and stained in Hematoxilin/Eosin or fixed in calcium formaldehyde and stained in mercury bromophenol blue.

Keywords
Phlebotominae - histology - historesin preparations

 
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