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Memórias do Instituto Oswaldo Cruz
Fundação Oswaldo Cruz, Fiocruz
ISSN: 1678-8060 EISSN: 1678-8060
Vol. 98, No. 2, 2003, pp. 287-290
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Bioline Code: oc03070
Full paper language: English
Document type: Research Article
Document available free of charge
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Memórias do Instituto Oswaldo Cruz, Vol. 98, No. 2, 2003, pp. 287-290
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SHORT COMMUNICATION - Preparation of Sand Fly (Diptera: Psychodidae: Phlebotominae) Specimens for Histological Studies
Beatriz Gomes Brazil; José Lino-Neto & Reginaldo Peçanha Brazil
Abstract
Phlebotominae sand fly specimens were prepared for histological and physiological
studies. Different fixatives were tested on sectioned and whole bodied adult
females in order to obtain good fixation and provide satisfactory penetration
of the embedding media. All fixed specimens were infiltrated (up to seven days
under 5ºC) and embedded in hydroxyethyl metacrylate. Two-three µm sections were stained, mounted in Canada balsam and observed by light microscopy.
Best results were achieved when whole bodied insects were double fixed in Bouin's
and Carnoy's fluids (4 h/2 h) and stained in Hematoxilin/Eosin or fixed in
calcium formaldehyde and stained in mercury bromophenol blue.
Keywords
Phlebotominae - histology - historesin preparations
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