Mycobacterium check for this species in other resources - livestock - bovine - polymerase chain reaction (PCR) - restriction fragment length polymorphism (RFLP) - mycolic acid analysis - Brazil"/>
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Memórias do Instituto Oswaldo Cruz
Fundação Oswaldo Cruz, Fiocruz
ISSN: 1678-8060
EISSN: 1678-8060
Vol. 98, No. 3, 2003, pp. 319-323
Bioline Code: oc03075
Full paper language: English
Document type: Research Article
Document available free of charge

Memórias do Instituto Oswaldo Cruz, Vol. 98, No. 3, 2003, pp. 319-323

 en Isolation and Identification of Mycobacteria from Livestock Specimens and Milk Obtained in Brazil
Clarice Q Fujimura Leite; Ivone S Anno; Sergio R de Andrade Leite; Eliana Roxo; Glenn P Morlock & Robert C Cooksey

Abstract

The prevalence of Mycobacterium bovis check for this species in other resources and other mycobacterial species in livestock specimens and milk was evaluated. An emphasis was placed upon the distribution of these organisms in milk that is readily available to the public that was either untreated, pasteurized, or treated using ultra high temperature. Twenty-two pathologic specimens from livestock (bovine, swine and bubaline) in five Brazilian states and 128 bovine milk samples from retail markets in the State of São Paulo were examined for mycobacteria. Identification was made by classical biochemical tests, thin layer chromatography of mycolic acids and polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) analysis. Mycobacteria were isolated from 15 (68.2%) caseous lesions and from 23 (18%) milk samples. Eleven isolates were identified as M. bovis, and the remaining 27 nontuberculous mycobacterial isolates were represented by five species and six unidentified rapidly growing mycobacterial strains. The data demonstrate that animal products in Brazil are frequent reservoirs of mycobacteria and may pose a risk to the public.

Keywords
Mycobacterium check for this species in other resources - livestock - bovine - polymerase chain reaction (PCR) - restriction fragment length polymorphism (RFLP) - mycolic acid analysis - Brazil

 
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