Trypanosoma cruzi check for this species in other resources - murine cardiac tissue"/>
search
for
 About Bioline  All Journals  Testimonials  Membership  News  Donations


Memórias do Instituto Oswaldo Cruz
Fundação Oswaldo Cruz, Fiocruz
ISSN: 1678-8060
EISSN: 1678-8060
Vol. 98, No. 3, 2003, pp. 373-376
Bioline Code: oc03083
Full paper language: English
Document type: Research Article
Document available free of charge

Memórias do Instituto Oswaldo Cruz, Vol. 98, No. 3, 2003, pp. 373-376

 en SHORT COMMUNICATION - Detection of Trypanosoma cruzi check for this species in other resources DNA within Murine Cardiac Tissue Sections by in Situ Polymerase Chain Reaction
Joshua E Lane; Rodrigo Ribeiro-Rodrigues; Danyvid Olivares-Villagómez; Cindy L Vnencak-Jones; Thomas L McCurley & Clint E Carter

Abstract

The use of in situ techniques to detect DNA and RNA sequences has proven to be an invaluable technique with paraffin-embedded tissue. Advances in non-radioactive detection systems have further made these procedures shorter and safer. We report the detection of Trypanosoma cruzi check for this species in other resources , the causative agent of Chagas disease, via indirect and direct in situ polymerace chain reaction within paraffin-embedded murine cardiac tissue sections. The presence of three T. cruzi specific DNA sequences were evaluated: a 122 base pair (bp) sequence localized within the minicircle network, a 188 bp satellite nuclear repetitive sequence and a 177 bp sequence that codes for a flagellar protein. In situ hybridization alone was sensitive enough to detect all three T. cruzi specific DNA sequences.

Keywords
in situ polymerase chain reaction (PCR) - indirect in situ PCR - direct in situ PCR - Trypanosoma cruzi check for this species in other resources - murine cardiac tissue

 
© Copyright 2003 - Instituto Oswaldo Cruz - Fiocruz
Alternative site location: http://memorias.ioc.fiocruz.br

Home Faq Resources Email Bioline
© Bioline International, 1989 - 2017, Site last up-dated on 16-Oct-2017.
Site created and maintained by the Reference Center on Environmental Information, CRIA, Brazil
System hosted by the Internet Data Center of Rede Nacional de Ensino e Pesquisa, RNP, Brazil