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Memórias do Instituto Oswaldo Cruz
Fundação Oswaldo Cruz, Fiocruz
ISSN: 1678-8060
EISSN: 1678-8060
Vol. 98, No. 3, 2003, pp. 391-393
Bioline Code: oc03088
Full paper language: English
Document type: Research Article
Document available free of charge

Memórias do Instituto Oswaldo Cruz, Vol. 98, No. 3, 2003, pp. 391-393

 en The Use of Polysiloxane/polyvinyl Alcohol Beads as Solid Phase in IgG Anti- Toxocara canis check for this species in other resources Detection Using a Recombinant Antigen
Raquel de Andrade Lima Coêlho; Hiroshi Yamasaki; Emília Perez & Luiz Bezerra de Carvalho Jr

Abstract

Immunodetection of human IgG anti- Toxocara canis check for this species in other resources was developed based on ELISA and on the use of polysiloxane/polyvinyl alcohol (POS/PVA) beads. A recombinant antigen was covalently immobilized, via glutaraldehyde, onto this hybrid inorganic-organic composite, which was prepared by the sol-gel technique. Using only 31.2 ng antigen per bead, a peroxidase conjugate dilution of 1:10,000 and a serum dilution of 1:200 were adequate for the establishment of the procedure. This procedure is comparable to that which utilizes the adsorption of the antigen to conventional PVC plates. However, the difference between positive and negative sera mean absorbances was larger for this new glass based assay. In addition to the performance of the POS/PVA bead as a matrix for immunodetection, its easy synthesis and low cost are additional advantages for commercial application.

Keywords
polysiloxane - polyvinyl alcohol - recombinant antigen - Toxocara canis check for this species in other resources

 
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