Trypanosoma cruzi check for this species in other resources - intracellular amastigotes - lysis - diterpenoids - Azorella compacta check for this species in other resources - chemotherapy - confocal microscopy"/>
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Memórias do Instituto Oswaldo Cruz
Fundação Oswaldo Cruz, Fiocruz
ISSN: 1678-8060
EISSN: 1678-8060
Vol. 98, No. 3, 2003, pp. 413-418
Bioline Code: oc03092
Full paper language: English
Document type: Research Article
Document available free of charge

Memórias do Instituto Oswaldo Cruz, Vol. 98, No. 3, 2003, pp. 413-418

 en Diterpenoids from Azorella compacta check for this species in other resources (Umbelliferae) Active on Trypanosoma cruzi check for this species in other resources
Jorge E Araya; Iván Neira; Solange da Silva; Renato A Mortara; Patricio Manque; Esteban Cordero; Hernán Sagua; Alberto Loyola; Jorge Bórquez; Glauco Morales & Jorge González

Abstract

The anti- Trypanosoma cruzi check for this species in other resources activity of natural products isolated from Azorella compacta check for this species in other resources was evaluated, with particular emphasis on their effect against intracellular amastigotes. Five diterpenoids from A. compacta derived from mulinane and azorellane were isolated and identified. Only two products, named azorellanol (Y-2) and mulin-11,3-dien-20-oic acid (Y-5), showed trypanocidal activity against all stages of T. cruzi including intracellular amastigotes. At 10 μM, these compounds displayed a strong lytic activity. It ranged from 88.4 ± 0.6 to 99.0 ± 1 % for all strains and stages evaluate, with an IC50 /18 h values of 20-84 μM and 41-87 μM, respectively. The development of intracellular amastigotes was also inhibited by nearly 60% at 25 μM. The trypanocidal molecules Y-2 and Y-5 did show different degrees of cytotoxicity depending on the cell line tested, with an IC50 /24 h ranging from 33.2 to 161.2 μM.

We evaluated the effect of diterpenoids against intracellular T. cruzi forms by immunofluorescent identification of a specific membrane molecular marker (Ssp-4 antigen) of the T. cruzi amastigote forms. The accuracy and reproducibility of the measurements were found to be outstanding when examined by confocal microscopy.

Keywords
Trypanosoma cruzi check for this species in other resources - intracellular amastigotes - lysis - diterpenoids - Azorella compacta check for this species in other resources - chemotherapy - confocal microscopy

 
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