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Memórias do Instituto Oswaldo Cruz
Fundação Oswaldo Cruz, Fiocruz
ISSN: 1678-8060
EISSN: 1678-8060
Vol. 98, No. 4, 2003, pp. 451-454
Bioline Code: oc03099
Full paper language: English
Document type: Research Article
Document available free of charge

Memórias do Instituto Oswaldo Cruz, Vol. 98, No. 4, 2003, pp. 451-454

 en Prevalence of Clostridium check for this species in other resources spp. and Clostridium difficile check for this species in other resources in Children with Acute Diarrhea in São Paulo City, Brazil
Claudia EA Ferreira; Viviane Nakano; Edison L Durigon & Mario J Avila-Campos

Abstract

Species of Clostridium check for this species in other resources are widely distributed in the environment, inhabiting both human and animal gastrointestinal tracts. Clostridium difficile check for this species in other resources is an important pathogen associated with outbreaks of pseudomembranous colitis and other intestinal disorders, such as diarrhea. In this study, the prevalence of Clostridium spp. and C. difficile, from hospitalized children with acute diarrhea, was examined. These children were admitted to 3 different hospitals for over 12 months. Eighteen (20%) and 19 (21%) stool specimens from children with (90) and without (91) diarrhea respectively, were positive to clostridia. Only 10 C. difficile strains were detected in 5.5% of the stool samples of children with diarrhea. None healthy children (without diarrhea) harbored C. difficile. From these 10 C. difficile, 9 were considered as toxigenic and genotyped as tcdA+/tcdB+ or tcdA-/tcdB+, and 1 strain as nontoxigenic (tcdA-/tdcB-). They were detected by the citotoxicity on VERO cells and by the multiplex-polymerase chain reaction. Thirty clinical fecal extracts produced minor alterations on VERO cells. The presence of C. difficile as a probable agent of acute diarrhea is suggested in several countries, but in this study, the presence of these organisms was not significant. More studies will be necessary to evaluate the role of clostridia or C. difficile in diarrhoeal processes in children.

Keywords
Clostridium difficile check for this species in other resources - acute diarrhea - toxins - diagnosis - São Paulo - Brazil

 
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