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Memórias do Instituto Oswaldo Cruz
Fundação Oswaldo Cruz, Fiocruz
ISSN: 1678-8060
EISSN: 1678-8060
Vol. 98, No. 4, 2003, pp. 465-468
Bioline Code: oc03102
Full paper language: English
Document type: Research Article
Document available free of charge

Memórias do Instituto Oswaldo Cruz, Vol. 98, No. 4, 2003, pp. 465-468

 en SHORT COMMUNICATION - Comparison between Specific and Multiplex Reverse Transcription-Polymerase Chain Reaction for Detection of Hepatitis A Virus, Poliovirus and Rotavirus in Experimentally Seeded Oysters
C Coelho; CEB Vinatea; AP Heinert; CMO Simões & CRM Barardi

Abstract

Outbreaks of gastroenteritis have occurred among consumers of raw or undercooked shellfish harvested from faecally polluted waters. A multiplex reverse transcription-polymerase chain reaction (RT-PCR) was applied for the simultaneous detection of hepatitis A virus (HAV), poliovirus (PV) and simian rotavirus (RV-SA11) and compared with specific primers for each genome sequence. Three amplified DNA products representing HAV (192 bp), PV (394 bp) and RV (278 bp) were identified when positive controls were used. However, when tested on experimentally contaminated raw oysters, this method was not able to detect the three viruses simultaneously. This is probably due to the low concentration of viral RNAs present in oyster extract which were partially lost during the extracts preparation.

Keywords
oysters - multiplex reverse transcription-polymerase chain reaction - poliovirus - rotavirus - hepatitis A virus

 
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