Random amplified polymorphic DNA (RAPD) markers were used to analyze 119 DNA samples of three Colombian Anopheles nuneztovari
populations to study genetic variation and structure. Genetic diversity, estimated from heterozygosity, averaged 0.34. Genetic flow was greater between the two populations located in Western Colombia (FST
: 0.035; Nm: 6.8) but lower between these two and the northeastern population (ΦST
: 0.08; Nm: 2.8). According to molecular variance analysis, the genetic distance between populations was significant (ΦST
0.1131, P < 0.001). The variation among individuals within populations (ΦST
0.8869, P < 0.001) was also significant, suggesting a greater degree of population subdivision, not considered in this study. Both the parameters evaluated and the genetic flow suggest that Colombian An. nuneztovari populations are co-specific.