Anopheles nuneztovari check for this species in other resources - genetic diversity - malaria vectors - Nyssorrhynchus check for this species in other resources - ramdon amplified polymorphic DNA - polymerase chain reaction - Colombia"/>
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Memórias do Instituto Oswaldo Cruz
Fundação Oswaldo Cruz, Fiocruz
ISSN: 1678-8060
EISSN: 1678-8060
Vol. 98, No. 4, 2003, pp. 469-476
Bioline Code: oc03103
Full paper language: English
Document type: Research Article
Document available free of charge

Memórias do Instituto Oswaldo Cruz, Vol. 98, No. 4, 2003, pp. 469-476

 en Random Amplified Polymorphic DNA Analysis of Anopheles nuneztovari check for this species in other resources (Diptera: Culicidae) from Western and Northeastern Colombia
Carmen Elisa Posso; Ranulfo González; Heiber Cárdenas; Gerardo Gallego; Myriam Cristina Duque & Marco Fidel Suarez

Abstract

Random amplified polymorphic DNA (RAPD) markers were used to analyze 119 DNA samples of three Colombian Anopheles nuneztovari check for this species in other resources populations to study genetic variation and structure. Genetic diversity, estimated from heterozygosity, averaged 0.34. Genetic flow was greater between the two populations located in Western Colombia (FST: 0.035; Nm: 6.8) but lower between these two and the northeastern population (ΦST: 0.08; Nm: 2.8). According to molecular variance analysis, the genetic distance between populations was significant (ΦST 0.1131, P < 0.001). The variation among individuals within populations (ΦST 0.8869, P < 0.001) was also significant, suggesting a greater degree of population subdivision, not considered in this study. Both the parameters evaluated and the genetic flow suggest that Colombian An. nuneztovari populations are co-specific.

Keywords
Anopheles nuneztovari check for this species in other resources - genetic diversity - malaria vectors - Nyssorrhynchus check for this species in other resources - ramdon amplified polymorphic DNA - polymerase chain reaction - Colombia

 
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