Trypanosoma cruzi check for this species in other resources - Leishmania check for this species in other resources - enzyme inhibitors - naphthoquinone"/>
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Memórias do Instituto Oswaldo Cruz
Fundação Oswaldo Cruz, Fiocruz
ISSN: 1678-8060
EISSN: 1678-8060
Vol. 98, No. 4, 2003, pp. 565-568
Bioline Code: oc03122
Full paper language: English
Document type: Research Article
Document available free of charge

Memórias do Instituto Oswaldo Cruz, Vol. 98, No. 4, 2003, pp. 565-568

 en 8-Methoxy-naphtho[2,3-b]thiophen-4,9-quinone, a Non-competitive Inhibitor of Trypanothione Reductase
Carlos L Zani & Alan H Fairlamb

Abstract

The enzyme trypanothione reductase is a recognised drug target in trypanosomatids and has been used in the search of new compounds with potential activity against diseases such as leishmaniasis, Chagas disease and African trypanosomiasis. 8-Methoxy-naphtho [2,3-b] thiophen-4,9-quinone was selected in a screening of natural and synthetic compounds using an in vitro assay with the recombinant enzyme from Trypanosoma cruzi. Its mode of inhibition fits a non-competitive model with respect to the substrate (trypanothione) and to the co-factor (NADPH), with Ki-values of 5 and 3.6 μM, respectively. When tested against human glutathione reductase, this compound did not display any significant inhibition at 100 μM, indicating a good selectivity against the parasite enzyme.

Keywords
trypanothione reductase - Trypanosoma cruzi check for this species in other resources - Leishmania check for this species in other resources - enzyme inhibitors - naphthoquinone

 
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