Trypanosoma cruzi check for this species in other resources - randomly amplified polymorphic DNA (RAPD) - simple sequence repeat-anchored polymerase chain reaction amplified (SSR-PCR) - glycoconjugates - biological characteristics"/>
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Memórias do Instituto Oswaldo Cruz
Fundação Oswaldo Cruz, Fiocruz
ISSN: 1678-8060
EISSN: 1678-8060
Vol. 98, No. 5, 2003, pp. 649-654
Bioline Code: oc03135
Full paper language: English
Document type: Research Article
Document available free of charge

Memórias do Instituto Oswaldo Cruz, Vol. 98, No. 5, 2003, pp. 649-654

 en Trypanosoma cruzi check for this species in other resources : Genetic Group with Peculiar Biochemical and Biological Behavior
Mônica Lúcia Gomes; Max Jean de Ornelas Toledo; Celso Vataru Nakamura; Nilza de Lucas Rodrigues Bittencourt; Egler Chiari & Silvana Marques de Araújo

Abstract

Thirty-two Trypanosoma cruzi check for this species in other resources strains, isolated from chronic chagasic patients in the northwest of the state of Paraná (Brazil), were analyzed using molecular, biochemical and biological characteristics. Genotypic analysis using randomly amplified polymorphic DNA and simple sequence repeat-anchored polymerase chain reaction amplified profiles showed a large, genetically well-correlated group that contained the majority of the strains and a divergent group that included the PR-150 strain. For glycoconjugate composition, the PR-150 strain was different from the other strains considering the absence or presence of specific bands in aqueous or detergent phases. This strain was also totally different from the others in one out of the six parameters related to in vitro and in vivo biological behavior. We highlight the fact that the PR-150 was totally resistant to benznidazole. For the other biological parameters this strain was not totally distinct from the others, but it showed a peculiar behavior.

Keywords
Trypanosoma cruzi check for this species in other resources - randomly amplified polymorphic DNA (RAPD) - simple sequence repeat-anchored polymerase chain reaction amplified (SSR-PCR) - glycoconjugates - biological characteristics

 
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