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Memórias do Instituto Oswaldo Cruz
Fundação Oswaldo Cruz, Fiocruz
ISSN: 1678-8060
EISSN: 1678-8060
Vol. 98, No. 5, 2003, pp. 655-658
Bioline Code: oc03136
Full paper language: English
Document type: Research Article
Document available free of charge

Memórias do Instituto Oswaldo Cruz, Vol. 98, No. 5, 2003, pp. 655-658

 en IS6110 Restriction Fragment Length Polymorphism of Mycobacterium tuberculosis check for this species in other resources Isolated from Patients with Pulmonary Tuberculosis in Campinas, Brazil. Evidence of Intercontinental Distribution of Strains
Ana Lucia Roscani Calusni; Gláucia Nogueira Roscani; Maria Cecília Barisson Villares; Hanna Soini; Edward A Graviss & Marcelo de Carvalho Ramos

Abstract

Tuberculosis (TB) is a major concern in developing countries. In Brazil, few genotyping studies have been conducted to verify the number of IS6110 copies present in local prevalent strains of Mycobacterium tuberculosis check for this species in other resources , the distribution and clustering of strains. IS6110 DNA fingerprinting was performed on a sample of M. tuberculosis isolates from patients with AFB smear-positive pulmonary TB, at a hospital in Brazil. The IS6110 profiles were analyzed and compared to a M. tuberculosis database of the Houston Tuberculosis Initiative, Houston, US. Seventy-six fingerprints were obtained from 98 patients. All M. tuberculosis strains had an IS6110 copy number between 5-21 allowing for differentiation of the isolates. Human immunodeficiency virus infection was confirmed in nearly half the patients of whom data was available. Fifty-eight strains had unique patterns, while 17 strains were grouped in 7 clusters (2 to 6 strains). When compared to the HTI database, 6 strains matched isolates from El Paso, Ciudad de Juarez, Houston, and New York. Recently acquired infections were documented in 19% of cases. The community transmission of infection is intense, since some clustered strains were recovered during the four-year study period. The intercontinental dissemination of M. tuberculosis strains is suspected by demonstration of identical fingerprints in a distant country.

Keywords
tuberculosis - Mycobacterium tuberculosis check for this species in other resources - IS6110 restriction fragment length polymorphism

 
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