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Memórias do Instituto Oswaldo Cruz
Fundação Oswaldo Cruz, Fiocruz
ISSN: 1678-8060
EISSN: 1678-8060
Vol. 98, No. 6, 2003, pp. 817-821
Bioline Code: oc03164
Full paper language: English
Document type: Research Article
Document available free of charge

Memórias do Instituto Oswaldo Cruz, Vol. 98, No. 6, 2003, pp. 817-821

 en Prevalence of cagA and vacA Genes in Isolates from Patients with Helicobacter pylori check for this species in other resources -associated Gastroduodenal Diseases in Recife, Pernambuco, Brazil
Carlos AA Brito; Lenôra MB Silva; Norma Jucá; Nilma C Leal; Wayner de Souza; Dulciene Queiroz; Francisco Cordeiro & Norma Lucena Silva

Abstract

Geographical differences in the prevalence of Helicobacter pylori check for this species in other resources genes and their association with disease severity have been identified. This study analyzes the prevalences of the cagA gene and alleles of the vacA gene in H. pylori-associated gastroduodenal diseases in isolates from Recife, PE, Brazil. Gastric biopsy of 61 H. pylori-positive patients were submitted to DNA extraction and gene amplification by polymerase chain reaction. Among the 61 patients, 21 suffered from duodenal ulcer (DU) and 40 from gastritis (GT). The prevalence of H. pylori strains harbouring the cagA gene was higher in the DU group (90.5%) than in the GT group (60%) (p = 0.02). The vacA gene was amplified in 56 out of 61 biopsies, of which 43 (76.8%) contained bacteria carrying the s1 allele and 13 (23.2%) the s2. However, the prevalence of the vacA s1 genotying was the same in either DU or GT group. The majority of the s1-typed strains, 39 (90.7%) out of 43, were subtype s1b. In resume there was a strong association between the H. pylori cagA+ gene and DU. However, there were no differences between the DU and GT groups in relation to the vacA s1 and s2 alleles distribution, albeit the subtype s1b was predominat.

Keywords
cagA - vacA- genotype - Helicobacter pylori check for this species in other resources - Recife - Brazil

 
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