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Memórias do Instituto Oswaldo Cruz
Fundação Oswaldo Cruz, Fiocruz
ISSN: 1678-8060
EISSN: 1678-8060
Vol. 98, No. 6, 2003, pp. 843-848
Bioline Code: oc03169
Full paper language: English
Document type: Research Article
Document available free of charge

Memórias do Instituto Oswaldo Cruz, Vol. 98, No. 6, 2003, pp. 843-848

 en Cytotoxicity and Potential Antiviral Evaluation of Violacein Produced by Chromobacterium violaceum check for this species in other resources
CR Andrighetti-Fröhner; RV Antonio; TB Creczynski-Pasa; CRM Barardi & CMO Simões

Abstract

Natural products are an inexhaustible source of compounds with promising pharmacological activities including antiviral action. Violacein, the major pigment produced by Chromobacterium violaceum, has been shown to have antibiotic, antitumoral and anti- Trypanosoma cruzi check for this species in other resources activities. The goal of the present work was to evaluate the cytotoxicity of violacein and also its potential antiviral properties.The cytotoxicity of violacein was investigated by three methods: cell morphology evaluation by inverted light microscopy and cell viability tests using the Trypan blue dye exclusion method and the MTT assay. The cytotoxic concentration values which cause destruction in 50% of the monolayer cells (CC50) were different depending on the sensitivity of the method. CC50 values were ≥ 2.07 ± 0.08 μM for FRhK-4 cells: ≥ 2.23 ± 0.11 μM for Vero cells; ≥ 2.54 ± 0.18 μM for MA104 cells; and ≥ 2.70 ± 0.20 μM for HEp-2 cells. Violacein showed no cytopathic inhibition of the following viruses: herpes simplex virus type 1 (HSV-1) strain 29-R/acyclovir resistant, hepatitis A virus (strains HM175 and HAF-203) and adenovirus type 5 nor did it show any antiviral activity in the MTT assay. However violacein did show a weak inhibition of viral replication: 1.42 ± 0.68%, 14.48 ± 5.06% and 21.47 ± 3.74% for HSV-1 (strain KOS); 5.96 ± 2.51%, 8.75 ± 3.08% and 17.75 ± 5.19% for HSV-1 (strain ATCC/VR-733); 5.13 ± 2.38 %, 8.18 ± 1.11% and 8.51 ± 1.94% for poliovirus type 2; 8.30 ± 4.24%; 13.33 ± 4.66% and 24.27 ± 2.18% for simian rotavirus SA11, at 0.312, 0.625 and 1.250 mM, respectively, when measured by the MTT assay.

Keywords
violacein - cytotoxicity - antiviral - MTT assay - herpes simplex virus type 1- poliovirus - rotavirus - hepatitis A virus - adenovirus

 
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