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Memórias do Instituto Oswaldo Cruz
Fundação Oswaldo Cruz, Fiocruz
ISSN: 1678-8060
EISSN: 1678-8060
Vol. 98, No. 6, 2003, pp. 849-851
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Bioline Code: oc03170
Full paper language: English
Document type: Research Article
Document available free of charge
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Memórias do Instituto Oswaldo Cruz, Vol. 98, No. 6, 2003, pp. 849-851
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SHORT COMMUNICATION - Strongyloides venezuelensis  Alkaline Extract for the Diagnosis of Human Strongyloidiasis by Enzyme-linked Immunosorbent Assay
Eleuza Rodrigues Machado; Marlene Tiduko Ueta; Maria do Rosário de Fátima Gonçalves-Pires; João Batista Alves de Oliveira; Lúcia Helena Faccioli & Julia Maria Costa-Cruz
Abstract
The present study was conducted to detected IgG antibodies using Strongyloides venezuelensis alkaline extract for the diagnosis of human strongyloidiasis by the enzyme-linked immunosorbent assay (ELISA). Sera from 90 subjects were analyzed (30 with strongyloidiasis, 30 with other parasites and 30 healthy individuals). Results were expressed in antibody titers, which were considered as positive when titer was ≥ 80. Sensibility and specificity of the assay were 100% and 96.7%, respectively. It can be concluded that the heterologous alkaline extract could be employed in ELISA as a diagnostic aid in human strongyloidiasis, due to its advantages as easiness of obtaining, practicability in preparing, and high indexes of sensitivity and specificity.
Keywords
Strongyloides venezuelensis - strongyloidiasis - diagnosis - enzyme-linked immunosorbent assay
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