Toxoplasma gondii check for this species in other resources - amylopectin granules - tissue cysts - ultrastructural cytochemistry"/>
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Memórias do Instituto Oswaldo Cruz
Fundação Oswaldo Cruz, Fiocruz
ISSN: 1678-8060
EISSN: 1678-8060
Vol. 98, No. 7, 2003, pp. 915-917
Bioline Code: oc03181
Full paper language: English
Document type: Research Article
Document available free of charge

Memórias do Instituto Oswaldo Cruz, Vol. 98, No. 7, 2003, pp. 915-917

 en An Alternative Technique to Reveal Polysaccharides in Toxoplasma gondii check for this species in other resources Tissue Cysts
Erick Vaz Guimarães; Laís de Carvalho & Helene Santos Barbosa

Abstract

Ultrathin sections of tissue cysts isolated from the brain of Toxoplasma gondii check for this species in other resources infected mice were submitted to two different methodologies derived from the periodic acid - Schiff's reagent (PAS) technique. The use of osmium tetroxide vapor as a developing agent of the aldehyde oxidation to reveal polysaccharides with periodic acid resulted in positive reaction in amylopectin granules in bradyzoites, as well as in the wall and matrix of the cysts, with excellent increment of the ultrastructural morphology. This technique can be used for study of T. gondii-host cell intracellular cycle, the differentiation tachyzoite-bradyzoite, and also for the formation of cysts into the host cells.

Keywords
Toxoplasma gondii check for this species in other resources - amylopectin granules - tissue cysts - ultrastructural cytochemistry

 
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