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Memórias do Instituto Oswaldo Cruz
Fundação Oswaldo Cruz, Fiocruz
ISSN: 1678-8060
EISSN: 1678-8060
Vol. 98, No. 8, 2003, pp. 1033-1038
Bioline Code: oc03202
Full paper language: English
Document type: Research Article
Document available free of charge

Memórias do Instituto Oswaldo Cruz, Vol. 98, No. 8, 2003, pp. 1033-1038

 en Preliminary Results of Random Amplification of Polymorphic DNA among Triatominae of the phyllosoma Complex (Hemiptera, Reduviidae)
Simone F Brenière; Bruno Taveira; Marie-France Bosseno; Rosalinda Ordoñez; Felipe Lozano-Kasten; Ezequiel Magallón-Gastélum; Ali Ouaissi & Janine Ramsey

Abstract

In Mexico, Triatoma longipennis check for this species in other resources (Usinger), Triatoma picturata check for this species in other resources (Usinger), and Triatoma pallidipennis check for this species in other resources (Stal), primary Chagas disease vector species of the phyllosoma complex, were analyzed by randomly amplified polymorphic DNA (RAPD). Sixteen decametric primers resolved individual profiles not identical, but partially discriminative between species. Analysis based on pairwise presence/absence comparisons between the three species was performed using three primers and two outgroup species Triatoma infestans check for this species in other resources (Klug) and Triatoma barberi check for this species in other resources (Usinger). Fifty-three bands in total were scored, although only two bands were constant among the three phyllosoma complex species. Two other bands were constant only for T. longipennis and T. picturata together, and not present in T. pallidipennis. Neighbor Joining tree and the multiple correspondence analysis discriminated T. pallidipennis clearly from the other two species, although there was overlap between T. longipennis and T. picturata. The results indicate a close relationship between the studied species and support the hypothesis of their recent evolution. The suitability of RAPD to discern populations within the species is discussed.

Keywords
Triatominae - phyllosoma complex - random amplification of polymorphic DNA - phylogeny - Mexico

 
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