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Memórias do Instituto Oswaldo Cruz
Fundação Oswaldo Cruz, Fiocruz
ISSN: 1678-8060
EISSN: 1678-8060
Vol. 98, No. 8, 2003, pp. 1097-1099
Bioline Code: oc03214
Full paper language: English
Document type: Research Article
Document available free of charge

Memórias do Instituto Oswaldo Cruz, Vol. 98, No. 8, 2003, pp. 1097-1099

 en SHORT COMMUNICATION - Detection of Cryptosporidium check for this species in other resources - Specific Coproantigen in Human Immunodeficiency Virus/Acquired Immunodeficiency Syndrome Patients by Using a Commercially Available Immunoenzymatic Assay
Claudio Vieira Silva; Marcelo Simão Ferreira; Maria do Rosário de Fátima Gonçalves-Pires & Julia Maria Costa-Cruz

Abstract

The aim of this study was to verify the occurrence of Cryptosporidium infection in 52 human immunodeficiency virus (HIV)/acquired immunodeficiency syndrome (AIDS) patients (group 1) and 38 clinically healthy individuals (group 2) by using enzyme immunoassay (EIA). All fecal samples collected were submitted to the Baermann, Lutz, and Ritchie methods, the Safranin/Methylene Blue, and Weber's chromotrope modified Trichrome staining techniques, and EIA. In group 1, parasitological staining techniques and EIA were both positive for Cryptosporidium check for this species in other resources sp. infection in 3/52 (5.8%) samples and both negative in 45/52 (86.5%) samples, while 4/52 (7.7%) samples were positive in EIA and negative in parasitological staining techniques. Concerning group 2, all samples were negative by EIA and microscopy for Cryptosporidium infection. In conclusion, EIA may be an alternative method for detecting Cryptosporidium-specific coproantigen in HIV/AIDS patients.

Keywords
Cryptosporidium check for this species in other resources sp. - coproantigen - acquired immunodeficiency syndrome (AIDS)

 
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