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Memórias do Instituto Oswaldo Cruz
Fundação Oswaldo Cruz, Fiocruz
ISSN: 1678-8060
EISSN: 1678-8060
Vol. 99, No. 2, 2004, pp. 153-158
Bioline Code: oc04028
Full paper language: English
Document type: Research Article
Document available free of charge

Memórias do Instituto Oswaldo Cruz, Vol. 99, No. 2, 2004, pp. 153-158

 en Analysis of the First and Second Internal Transcribed Spacer Sequences of the Ribosomal DNA in Biomphalaria tenagophila check for this species in other resources Complex (Mollusca: Planorbidae)
Teofânia HDA Vidigal Linus Spatz; Jessica C Kissinger; Rodrigo AF Redondo; Edina CR Pires; Andrew JG Simpson & Omar S Carvalho

Abstract

The first and second internal transcribed spacer regions (ITS1 and ITS2) of the ribosomal DNA of Biomphalaria tenagophila check for this species in other resources complex (B. tenagophila, B. occidentalis, and B. t. guaibensis) were sequenced and compared. The alignment lengths of these regions were about 655 bp and 481 bp, respectively. Phylogenetic relationships among the Biomphalaria check for this species in other resources species were inferred by Maximum Parsimony and Neighbor-joining methods. The phylogenetic trees produced, in most of the cases, were in accordance with morphological systematics and other molecular data previously obtained by polymerase chain reaction and restriction fragment length polymorphism analysis. The present results provide support for the proposal that B. tenagophila represents a complex comprising B. tenagophila, B. occidentalis and B. t. guaibensis.

Keywords
Biomphalaria - snails - ribosomal DNA - internal transcribed spacer - polymerase chain reaction

 
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