The first and second internal transcribed spacer regions (ITS1 and ITS2) of the ribosomal DNA of
Biomphalaria tenagophila
complex (
B. tenagophila,
B. occidentalis, and
B. t. guaibensis) were sequenced and compared. The alignment lengths of these regions were about 655 bp and 481 bp, respectively. Phylogenetic relationships among the
Biomphalaria
species were inferred by Maximum Parsimony and Neighbor-joining methods. The phylogenetic trees produced, in most of the cases, were in accordance with morphological systematics and other molecular data previously obtained by polymerase chain reaction and restriction fragment length polymorphism analysis. The present results provide support for the proposal that
B. tenagophila represents a complex comprising
B. tenagophila,
B. occidentalis and
B. t. guaibensis.
