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Memórias do Instituto Oswaldo Cruz
Fundação Oswaldo Cruz, Fiocruz
ISSN: 1678-8060
EISSN: 1678-8060
Vol. 99, No. 2, 2004, pp. 179-184
Bioline Code: oc04032
Full paper language: English
Document type: Research Article
Document available free of charge

Memórias do Instituto Oswaldo Cruz, Vol. 99, No. 2, 2004, pp. 179-184

 en Stage-specific Activity of Potential Antimalarial Compounds Measured in Vitro by Flow Cytometry in Comparison to Optical Microscopy and Hypoxanthine Uptake
Carmen E Contreras; María A Rivas; José Domínguez; Jaime Charris; Mario Palacios; Nicolás E Bianco & Isaac Blanca

Abstract

The evaluation of new antimalarial agents using older methods of monitoring sensitivity to antimalarial drugs are laborious and poorly suited to discriminate stage-specific activity. We used flow cytometry to study the effect of established antimalarial compounds, cysteine protease inhibitors, and a quinolone against asexual stages of Plasmodium falciparum check for this species in other resources . Cultured P. falciparum parasites were treated for 48 h with different drug concentrations and the parasitemia was determined by flow cytometry methods after DNA staining with propidium iodide. P. falciparum erythrocytic life cycle stages were readily distinguished by flow cytometry. Activities of established and new antimalarial compounds measured by flow cytometry were equivalent to results obtained with microscopy and metabolite uptake assays. The antimalarial activity of all compounds was higher against P. falciparum trophozoite stages. Advantages of flow cytometry analysis over traditional assays included higher throughput for data collection, insight into the stage-specificity of antimalarial activity avoiding use of radioactive isotopes.

Keywords
Plasmodium falciparum - antimalarial compounds - flow cytometry - hypoxanthine uptake - chloroquine - quinolone

 
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