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Memórias do Instituto Oswaldo Cruz
Fundação Oswaldo Cruz, Fiocruz
ISSN: 1678-8060
EISSN: 1678-8060
Vol. 99, No. 2, 2004, pp. 189-193
Bioline Code: oc04034
Full paper language: English
Document type: Research Article
Document available free of charge

Memórias do Instituto Oswaldo Cruz, Vol. 99, No. 2, 2004, pp. 189-193

 en Immunoblotting for the Serodiagnosis of Helicobacter pylori check for this species in other resources Infection in Brazilian Patients with and without Gastric Carcinoma
Andreia MC Rocha; Gifone A Rocha; Janise L Leite; Renato LL Lisboa; Patrícia VO Silva & Dulciene M M Queiroz


We evaluated the performance of a commercial immunoblotting in the serodiagnosis of Helicobacter pylori check for this species in other resources infection in Brazilian patients. The presence of anti-H. pylori antibodies was also investigated in a group of 20 duodenal ulcer patients after successful treatment. One hundred and ninety one patients were studied. Among the 164 infected patients, 46 had gastric carcinoma. The duodenal ulcer patients were treated with antimicrobial drugs and the eradication of the microorganism was confirmed in all of them one month after the end of the treatment by the 13C-urea breath test. Sera were assayed for H. pylori antibodies using the Helicoblot 2.0 (Genelabs Diagnostics, Singapore). The sensitivity, specificity, positive, and negative predictive values of the test were 93.9%, 92.6%, 98.7%, and 71.4%, respectively. The sensitivity of the test was similar in patients with (93.5%) and without (95.7%) gastric carcinoma. Twenty-four months after the end of the treatment, the band of 116 kDa was still detected in one of the patients. In conclusion, the Helicoblot 2.0 is an accurate test to diagnose H. pylori infection and although it can not be employed to monitor the bacterium eradication, it may be useful for diagnosing past infection, especially in gastric carcinoma patients.

Helicobacter pylori - H. pylori/diagnosis - immunoblotting

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