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Memórias do Instituto Oswaldo Cruz
Fundação Oswaldo Cruz, Fiocruz
ISSN: 1678-8060
EISSN: 1678-8060
Vol. 99, No. 4, 2004, pp. 415-419
Bioline Code: oc04073
Full paper language: English
Document type: Research Article
Document available free of charge

Memórias do Instituto Oswaldo Cruz, Vol. 99, No. 4, 2004, pp. 415-419

 en Standardization of in-house Polymerase Chain Reaction for the Identification of Mycobacterium tuberculosis check for this species in other resources at the Reference Tropical Disease Hospital in the State of Goiás, Brazil
Márcia Alves Vasconcelos Rodrigues; Álvaro Bisol Serafini; Marieta de Souza Pereira; Thathiane Dias da Silva; Marcelo Fouad Rabahi; Suely Lemes de Alves & André Kipnis

Abstract

This study compares smear, growth in Lowenstein-Jensen medium, and in-house polymerase chain reaction (PCR) techniques for the detection of Mycobacterium tuberculosis check for this species in other resources . A total of 72 specimens from 72 patients with clinical symptoms of tuberculosis, including 70 sputum and two bronchial aspirate samples, were tested in parallel by smear, culture, and in-house PCR techniques. From these, 48 (66.6%) were negative by the 3 methods, 2 (2.8%) were smear positive and negative by culture and in-house PCR, 11 (15.3%) were both smear and culture negative, and in-house PCR positive, 7 (9.7%) were positive by the 3 methods, 2 (2.8%) were positive by smear and culture, and negative by PCR, 2 (2.8%) were positive by culture and PCR, but smear negative. After the resolution of discrepancies in PCR results, the sensitivity and specificity for in-house PCR technique to M. tuberculosis relative to the culture, were 81.8% and 81.9%, respectively. These results confirm that this method, in-house PCR, may be a sensitive and specific technique for M. tuberculosis detection, occurring in both positive and negative smear and negative cultures.

Keywords
Mycobacterium tuberculosis - polymerase chain reaction - tuberculosis - culture - smear

 
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