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Memórias do Instituto Oswaldo Cruz
Fundação Oswaldo Cruz, Fiocruz
ISSN: 1678-8060
EISSN: 1678-8060
Vol. 99, No. 4, 2004, pp. 421-424
Bioline Code: oc04074
Full paper language: English
Document type: Research Article
Document available free of charge

Memórias do Instituto Oswaldo Cruz, Vol. 99, No. 4, 2004, pp. 421-424

 en Detection of Lymnaea columella check for this species in other resources Infection by Fasciola hepatica check for this species in other resources through Multiplex-PCR
Kelly Grace Magalhães; Liana Konovaloff Jannotti Passos & Omar dos Santos Carvalho

Abstract

From complete mitochondrial DNA sequence of Fasciola hepatica check for this species in other resources available in Genbank, specific primers were designed for a conserved and repetitive region of this trematode. A pair of primers was used for diagnosis of infected Lymnaea columella check for this species in other resources by F. hepatica during the pre-patent period simultaneously with another pair of primers which amplified the internal transcribed spacer (ITS) region of rDNA from L. columella in a single Multiplex-PCR. The amplification generated a ladder band profile specific for F. hepatica . This profile was observed in positive molluscs at different times of infection, including adult worms from the trematode. The Multiplex-PCR technique showed to be a fast and safe tool for fascioliasis diagnosis, enabling the detection of F. hepatica miracidia in L. columella during the pre-patent period and identification of transmission areas.

Keywords
Fasciola hepatica - Lymnaea columella - multiplex polymerase chain reaction - DNA mitochondrial - diagnosis

 
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