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Memórias do Instituto Oswaldo Cruz
Fundação Oswaldo Cruz, Fiocruz
ISSN: 1678-8060
EISSN: 1678-8060
Vol. 99, No. 5, 2004, pp. 503-507
Bioline Code: oc04108
Full paper language: English
Document type: Research Article
Document available free of charge

Memórias do Instituto Oswaldo Cruz, Vol. 99, No. 5, 2004, pp. 503-507

 en The Use of the Polymerase Chain Reaction and Restriction Fragment Length Polymorphism Technique Associated with the Classical Morphology for Characterization of Lymnaea columella check for this species in other resources , L. viatrix check for this species in other resources , and L. diaphana check for this species in other resources (Mollusca: Lymnaeidae)
Omar S Carvalho; Paula CM Cardoso; Pollanah M Lira; Alejandra Rumi; Andrea Roche; Elizabeth Berne; Gertrud Müller & Roberta L Caldeira

Abstract

The specific identification of Lymnaeid snails is based on a comparison of morphological characters of the shell, radula, renal and reproductive organs. However, the identification is complicated by dissection process, intra and interspecific similarity and variability of morphological characters. In the present study, polymerase chain reaction and restriction fragment length polymorphism (PCR-RFLP) techniques targeted to the first and second internal transcribed spacers (ITS1 and ITS2) rDNA and to the mitochondrial 16S ribosomal gene (16S rDNAmt) were used to differentiate the species Lymnaea columella check for this species in other resources , L. viatrix, and L. diaphana from some localities of Brazil, Argentina, and Uruguay as well as to verify whether the molecular results corroborates the classical morphological method. PCR-RFLP analysis of the ITS1, ITS2, and 16S using 12 restriction enzymes revealed characteristic patterns for L. columella and L. diaphana which were concordant with the classical morphology. On the other hand, for L. viatrix populations a number of 1 to 6 profiles were generated while morphology provided the species pattern results.

Keywords
Lymnaea columella - Lymnaea viatrix - Lymnaea diaphana - polymerase chain reaction and restriction fragment length polymorphism - first and second internal transcribed spacers of the RNA ribosomal gene - subunit 16S of mitochondrial RNA ribosomal gene

 
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