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Memórias do Instituto Oswaldo Cruz
Fundação Oswaldo Cruz, Fiocruz
ISSN: 1678-8060
EISSN: 1678-8060
Vol. 99, No. 5, 2004, pp. 513-516
Bioline Code: oc04110
Full paper language: English
Document type: Research Article
Document available free of charge

Memórias do Instituto Oswaldo Cruz, Vol. 99, No. 5, 2004, pp. 513-516

 en SHORT COMMUNICATION - HygR and PurR Plasmid Vectors for Episomal Transfection of Trypanosoma cruzi check for this species in other resources
Otávio T Nóbrega; Jaime M Santana; Nancy R Sturm; Antônio RL Teixeira & David A Campbell

Abstract

This work describes the development and functional testing of two episomes for stable transfection of Trypanosoma cruzi check for this species in other resources . pHygD contained the 5'- and 3'- flanking regions of the gene encoding the cathepsin B-like protease of T. cruzi as functional trans-splicing and polyadenylation signals for the hygR ORF. Evidence is presented to support extrachromosomal maintenance and organization as tandem repeats in transfected parasites. pPac was derived from pHygD by replacement of the entire hygR ORF with a purR coding region. The ability to modify pHygD and the availability of the complete DNA sequence make these plasmids useful tools for the genetic manipulation of T. cruzi.

Keywords
Trypanosoma cruzi - selectable markers - stable transfection

 
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