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Memórias do Instituto Oswaldo Cruz
Fundação Oswaldo Cruz, Fiocruz
ISSN: 1678-8060
EISSN: 1678-8060
Vol. 99, No. 7, 2004, pp. 697-701
Bioline Code: oc04142
Full paper language: English
Document type: Research Article
Document available free of charge

Memórias do Instituto Oswaldo Cruz, Vol. 99, No. 7, 2004, pp. 697-701

 en In Vitro Measurement of Enzymatic Markers as a Tool to Detect Mouse Cardiomyocytes Injury
Elen Mello de Souza; Andrea Henriques-Pons; Christian Bailly; Amelie Lansiaux; Tânia Cremonine Araújo-Jorge & Maria de Nazaré Correia Soeiro

Abstract

Primary cultures of cardiomyocytes represent a useful model for analyzing cardiac cell biology as well as pathogenesis of several cardiovascular disorders. Our aim was to standardize protocols for determining the damage of cardiac cells cultured in vitro by measuring the creatine kinase and its cardiac isotype and lactate dehydrogenase activities in the supernatants of mice cardiomyocytes submitted to different protocols of cell lysis. Our data showed that due to its higher specificity, the cardiac isotype creatine kinase was the most sensitive as compared to the others studied enzymatic markers, and can be used to monitor and evaluate cardiac damage in in vitro assays.

Keywords
creatine kinase - in vitro assays - cardiac damage

 
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