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Memórias do Instituto Oswaldo Cruz
Fundação Oswaldo Cruz, Fiocruz
ISSN: 1678-8060
EISSN: 1678-8060
Vol. 99, No. 8, 2004, pp. 895-897
Bioline Code: oc04175
Full paper language: English
Document type: Research Article
Document available free of charge

Memórias do Instituto Oswaldo Cruz, Vol. 99, No. 8, 2004, pp. 895-897

 en Short Communication - Hepatitis C Virus Genotypes in Blood Donors from the Federal District, Central Brazil
Regina MS Amorim Claudiner P Oliveira; Patrícia S Wyant; Daniela M Cerqueira; Geni NL Câmara; Luciano S Flores; Regina MB Martins & Cláudia RF Martins

Abstract

The objective of this study was to characterize hepatitis C virus (HCV) genotypes in blood donors from the Federal District, Central Brazil, and to compare HCV screening by serological assays and reverse transcriptase polymerase chain reaction (RT-PCR). Plasma samples from 57 individuals with reactive or indeterminate results in serological anti-HCV screening assays (ELISA or EIA) were tested for HCV RNA by RT-PCR. The results from a confirmatory LIA serological assay were also evaluated. The 5' non-coding region of the HCV genome was amplified from 41 PCR positive samples (71.9%), which were further characterized by nucleotide sequencing analysis. Of these, 60.9% were of HCV genotype 1 and 39.1% of genotype 3.

Keywords
hepatitis C virus - genotypes - Federal District, Central Brazil

 
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