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Memórias do Instituto Oswaldo Cruz
Fundação Oswaldo Cruz, Fiocruz
ISSN: 1678-8060
EISSN: 1678-8060
Vol. 100, No. 1, 2005, pp. 19-23
Bioline Code: oc05004
Full paper language: English
Document type: Research Article
Document available free of charge

Memórias do Instituto Oswaldo Cruz, Vol. 100, No. 1, 2005, pp. 19-23

 en Biomphalaria tenagophila check for this species in other resources - dominant resistance character - Schistosoma mansoni check for this species in other resources - crossbreedings
Florence Mara Rosa; Ana Lúcia Brunialti Godard; Vasco Azevedo & Paulo Marcos Zech Coelho

Abstract

The aim of the present work was to study parasitological, molecular, and genetic aspects in descendants of crossbreedings between a totally resistant Biomphalaria tenagophila check for this species in other resources strain (Taim, RS) and another one highly susceptible (Joinville, SC) to Schistosoma mansoni check for this species in other resources . Descendants F1 and F2 were submitted to S. mansoni infection (LE strain). The susceptibility rates for individuals from Group F1 were 0 to 0.6%, and from Group F2 was 7.2%. The susceptible individuals from Group F2 discharged a lower number of cercariae, when compared with the susceptible parental group, and in 2 out of 9 positive snails the cercarial elimination was discontinued. In order to identify genetic markers associated with resistance the genotype of parental snails and their offspring F1 and F2 were analyzed by means of the randomly amplified polymorphic DNA method. Nevertheless, it was not possible to detect any marker associated to resistance, but the results showed that in the mentioned species the resistance character is determined by two dominant genes.

Keywords
Biomphalaria tenagophila - dominant resistance character - Schistosoma mansoni - crossbreedings

 
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