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Memórias do Instituto Oswaldo Cruz
Fundação Oswaldo Cruz, Fiocruz
ISSN: 1678-8060
EISSN: 1678-8060
Vol. 100, No. 1, 2005, pp. 33-38
Bioline Code: oc05006
Full paper language: English
Document type: Research Article
Document available free of charge

Memórias do Instituto Oswaldo Cruz, Vol. 100, No. 1, 2005, pp. 33-38

 en Programmed cell death in Trypanosoma cruzi check for this species in other resources induced by Bothrops jararaca check for this species in other resources venom
Poliana Deolindo; André S. Teixeira-Ferreira; Edésio JT Melo; Andrea Cristina Veto Arnholdt; Wanderley de Souza; Elias W Alves & Renato A DaMatta

Abstract

Cells die through a programmed process or accidental death, know as apoptosis or necrosis, respectively. Bothrops jararaca check for this species in other resources is a snake whose venom inhibits the growth of Trypanosoma cruzi check for this species in other resources epimastigote forms causing mitochondrion swelling and cell death. The aim of the present work was to determine the type of death induced in epimastigotes of T. cruzi by this venom. Parasite growth was inhibited after venom treatment, and 50% growth inhibition was obtained with 10 μg/ml. Ultrastructural observations confirmed mitochondrion swelling and kinetoplast disorganization. Furthermore, cytoplasmic condensation, loss of mitochondrion membrane potential, time-dependent increase in phosphatidylserine exposure at the outer leaflet plasma membrane followed by permeabilization, activation of caspase like protein and DNA fragmentation were observed in epimastigotes throughout a 24 h period of venom treatment. Taken together, these results indicate that the stress induced in epimastigote by this venom, triggers a programmed cell death process, similar to metazoan apoptosis, which leads to parasite death.

Keywords
Trypanosoma cruzi - programmed cell death -Bothrops jararaca - snake venom

 
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