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Memórias do Instituto Oswaldo Cruz
Fundação Oswaldo Cruz, Fiocruz
ISSN: 1678-8060
EISSN: 1678-8060
Vol. 100, No. 1, 2005, pp. 43-46
Bioline Code: oc05008
Full paper language: English
Document type: Research Article
Document available free of charge

Memórias do Instituto Oswaldo Cruz, Vol. 100, No. 1, 2005, pp. 43-46

 en Multiplex-PCR for detection of natural Leishmania infection in Lutzomyia check for this species in other resources spp. captured in an endemic region for cutaneous leishmaniasis in state of Sucre, Venezuela
Alicia Jorquera; Ricardo González; Edgar Marchán-Marcano; Milagros Oviedo & Mercedes Matos

Abstract

We studied the natural infection of Lutzomyia check for this species in other resources (Lutzomyia) sp. with Leishmania in endemic foci of cutaneous leishmaniasis in the Paria peninsula, state of Sucre, Venezuela. Sand flies were collected between March 2001 and June 2003, using Shannon light-traps and human bait. Of the 1291 insects captured, only two species of phlebotomines were identified: L. ovallesi (82.75%) and L. gomezi (17.42%). A sample of the collected sand flies (51 pools of 2-12 individuals) were analyzed by using a multiplex-PCR assay for simultaneous detection of New Word Leishmania and Viannia subgenera. The results showed a total of 8 pools (15.68%) infected; of these, 7 were L. ovallesi naturally infected with L. braziliensis (2 pools) and L. mexicana (5 pools) and 1 pool of L. gomezi infected by L. braziliensis.

Keywords
cutaneous leishmaniasis - sand flies vectors - natural infections - polymerase chain reaction - Lutzomyia spp. - Venezuela

 
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