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Memórias do Instituto Oswaldo Cruz
Fundação Oswaldo Cruz, Fiocruz
ISSN: 1678-8060
EISSN: 1678-8060
Vol. 100, No. 3, 2005, pp. 241-243
Bioline Code: oc05076
Full paper language: English
Document type: Research Article
Document available free of charge

Memórias do Instituto Oswaldo Cruz, Vol. 100, No. 3, 2005, pp. 241-243

 en Short Communication- Trypanosoma rangeli check for this species in other resources sialidase: lack of activity in stocks from Panama and other regions
Octavio E. Sousa; Maritza Lombardo & Azael Saldaña

Abstract

A total of 33 crude and cloned Trypanosoma rangeli check for this species in other resources stocks found as natural infections in human from Panama and other endemic areas of Central and South America were evaluated as producers of sialidase (SA) activity through the MU-NANA fluorescence test. Negative results were observed in 6 of the isolates: Panama (4), Honduras (1), and Brazil (1). In addition, an immunoblotting analysis confirm the presence of the SA antigen in these stocks without enzymatic activity. These findings must be considered in the interpretation of the biological significance of T. rangeli SA and in the proper characterization and identification of this parasite.

Keywords
Trypanosoma rangeli - sialidase - heterogeneity

 
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