en |
A follow up study of cytomegalovirus infection in a group of Turkish renal transplant recipients using molecular assays
Meltem Yalinay Cirak; Seyyal Rota; Isil Maral; Sevgi Turet & Sukru Sindel
Abstract
The clinical
value of an in-house cytomegalovirus nested polymerase chain reaction (CMV-PCR)
and a commercial molecular assay hybrid capture CMV DNA assay (HCA) was
evaluated in monitoring a group of renal transplant patients for six months
follow up. In this study, the sensitivity, specificity, positive predictive
value, and negative predictive value of nested CMV DNA PCR assay and HCA
at the beginning of the study were 70, 42.9, 46.7, 66.7, and 60, 78.6,
66.7, and 73.3% respectively. After six months, they were 80, 66.7, 80,
66.7 for CMV PCR and 73.3, 88.9, 91.7, 66.7% for HCA respectively. These
results indicate that in monitoring and predicting CMV infections in renal
transplant recipients, not only qualitative but also quantitative assays
must be used together in order to decide the preemptive strategies.
Keywords
cytomegalovirus - polymerase chain reaction - hybridization
|