Brazilian spotted fever (BSF) cases have been increasing in the state of São Paulo but no genomic information about local rickettsia isolated from humans has been well documented. We recovered spotted-fever group rickettsiae from a sample of patient blood cultured in Vero cells using the shell vial technique. Rickettsial DNA fragments (glt
A, and, omp
B genes) were detected, and analysis of the ompB gene base sequences showed identity with the Rickettsia rickettsii
ompB sequence available in the GenBank.