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Memórias do Instituto Oswaldo Cruz
Fundação Oswaldo Cruz, Fiocruz
ISSN: 1678-8060
EISSN: 1678-8060
Vol. 100, No. 3, 2005, pp. 277-279
Bioline Code: oc05083
Full paper language: English
Document type: Research Article
Document available free of charge

Memórias do Instituto Oswaldo Cruz, Vol. 100, No. 3, 2005, pp. 277-279

 en Short Communication - Detection of Brazilian spotted fever infection by polymerase chain reaction in a patient from the state of São Paulo
Elvira Maria Mendes Nascimento; Flávia de Sousa Gehrke; Rosa Amélia Maldonado; Silvia Colombo; Luiz Jacintho da Silva & Teresinha Tizu Sato Schumaker

Abstract

Brazilian spotted fever (BSF) cases have been increasing in the state of São Paulo but no genomic information about local rickettsia isolated from humans has been well documented. We recovered spotted-fever group rickettsiae from a sample of patient blood cultured in Vero cells using the shell vial technique. Rickettsial DNA fragments (gltA, ompA, and, ompB genes) were detected, and analysis of the ompB gene base sequences showed identity with the Rickettsia rickettsii check for this species in other resources ompB sequence available in the GenBank.

Keywords
Brazilian spotted fever - Rickettsia rickettsii - ompB gene

 
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