We compared
the in vitro growth of promastigotes from two
Leishmania species
in TC-100 and Schneider media.
Leihsmania
(Leishmania) amazonensis
replication
rates were similar in both tissue culture media and reached maximum rates
by 48 h. In contrast
Leishmania (Viannia) braziliensis
growth was
significantly greater in TC-100 but maximum rates were achieved by 96 h.
Folic acid appears to be the limiting factor and supplementation of Schneider
media with this nutrient improved
L. (V.) braziliensis replication
rates and decreased the time of maximum replication to 48 h.
