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Memórias do Instituto Oswaldo Cruz
Fundação Oswaldo Cruz, Fiocruz
ISSN: 1678-8060
EISSN: 1678-8060
Vol. 100, No. 4, 2005, pp. 385-389
Bioline Code: oc05101
Full paper language: English
Document type: Research Article
Document available free of charge

Memórias do Instituto Oswaldo Cruz, Vol. 100, No. 4, 2005, pp. 385-389

 en Analysis of expressed sequence tags from Trypanosoma cruzi check for this species in other resources amastigotes
Gustavo C. Cerqueira; Wanderson D. Da Rocha; Priscila C. Campos; Cláudia S. Zouain & Santuza M. R. Teixeira

Abstract

A total of 880 expressed sequence tags (EST) originated from clones randomly selected from a Trypanosoma cruzi check for this species in other resources amastigote cDNA library have been analyzed. Of these, 40% (355 ESTs) have been identified by similarity to sequences in public databases and classified according to functional categorization of their putative products. About 11% of the mRNAs expressed in amastigotes are related to the translational machinery, and a large number of them (9% of the total number of clones in the library) encode ribosomal proteins. A comparative analysis with a previous study, where clones from the same library were selected using sera from patients with Chagas disease, revealed that ribosomal proteins also represent the largest class of antigen coding genes expressed in amastigotes (54% of all immunoselected clones). However, although more than thirty classes of ribosomal proteins were identified by EST analysis, the results of the immunoscreening indicated that only a particular subset of them contains major antigenic determinants recognized by antibodies from Chagas disease patients.

Keywords
Trypanosoma cruzi - gene expression - amastigotes - antigens

 
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