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Memórias do Instituto Oswaldo Cruz
Fundação Oswaldo Cruz, Fiocruz
ISSN: 1678-8060
EISSN: 1678-8060
Vol. 100, No. 4, 2005, pp. 397-401
Bioline Code: oc05103
Full paper language: English
Document type: Research Article
Document available free of charge

Memórias do Instituto Oswaldo Cruz, Vol. 100, No. 4, 2005, pp. 397-401

 en Biological effects of extracts obtained from Stryphnodendron adstringens check for this species in other resources on Herpetomonas samuelpessoai check for this species in other resources
Fabíola Barbieri Holetz; Tânia Ueda-Nakamura; Benedito Prado Dias Filho; João Carlos Palazzo de Mello; José Andrés Morgado-Díaz; Cleyton Eduardo Mendes de Toledo & Celso Vataru Nakamura

Abstract

We report the effect of Stryphnodendron adstringens check for this species in other resources on the trypanosomatid Herpetomonas samuelpessoai check for this species in other resources . The parasites were grown at 28°C in a chemically defined medium containing crude extract and fractions at concentrations from 100 to 5000 μg/ml obtained from S. adstringens. Concentrations of 500, 1000, 2500, and 5000 μg/ml both crude extract and semi-purified fraction progressively inhibited the protozoans´ growth. At a concentration of 100 μg/ml, crude extract or a semi-purified (F3) fraction did not affect the growth of the protozoans. The F3-9 - F3-12 sub-fractions, at a concentration of 1000 μg/ml, also showed increased inhibitory activity on H. samuelpessoai. The IC50 of the crude extract and the F3 fraction were 538 and 634 μg/ml, respectively. Ultrastructural and enzymatic alterations in the trypanosomatids were also evaluated. H. samuelpessoai cultivated in the presence of IC50 crude extract showed considerable ultrastructural alterations, such as marked mitochondrial swelling with a large number of cristae and evident Golgi complex vesiculation, as observed by transmission electron microscopy. Cells exposed to 538 μg/ml of crude extract at 28°C for 72 h, showed decreased activity of the enzyme succinate cytochrome c reductase, a typical mitochondrion marker, as compared to untreated cells

Keywords
Herpetomonas samuelpessoai - Stryphnodendron adstringens - ultrastructural alteration - antiprotozoan activity

 
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