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Memórias do Instituto Oswaldo Cruz
Fundação Oswaldo Cruz, Fiocruz
ISSN: 1678-8060
EISSN: 1678-8060
Vol. 100, No. 6, 2005, pp. 613-617
Bioline Code: oc05134
Full paper language: English
Document type: Research Article
Document available free of charge

Memórias do Instituto Oswaldo Cruz, Vol. 100, No. 6, 2005, pp. 613-617

 en Serological survey of Babesia bovis check for this species in other resources , Babesia bigemina check for this species in other resources , and Anaplasma marginale check for this species in other resources antibodies in cattle from the semi-arid region of the state of Bahia, Brazil, by enzyme-linked immunosorbent assays
Silvia L Barros; Claudio R Madruga; Flabio R Araújo; Carlos F Menk; Maria Angela O de Almeida; Elaine PS Melo & Raul H Kessler

Abstract

The objectives of this work were to determine the prevalence of Babesia bovis check for this species in other resources , Babesia bigemina check for this species in other resources , and Anaplasma marginale check for this species in other resources detecting antibodies in cattle raised in the semi-arid region of the state of Bahia, Brazil, through indirect enzyme linked immunosorbent assays (ELISA) and to compare the performances of indirect enzyme-linked immunosorbent assays with crude (I-ELISA-CrAnaAg) and recombinant major surface protein-5 (I-ELISA-MSP-5Ag), as antigens to detect antibodies against A. marginale. An stable enzootic area was found in Senhor do Bonfim and Euclides da Cunha for B. bovis that showed 86 and 95.5% of prevalence, respectively, and for B. bigemina with 90.8 and 91.3%. On the other hand, Uauá and Juazeiro, were characterized as enzootically unstable areas, since prevalences were: B. bovis - 63.7 and 56.4% and B. bigemina - 53 and 54.8%, respectively. The prevalence of A. marginale in the four municipalities was above 97% with I-ELISA-CrAnaAg and 94.8% with I-ELISA-MSP-5Ag which is an indication of stable enzootic condition for the rickettsia. The I-ELISA-CrAnaAg and I-ELISA-MSP-5Ag showed a highly significant association (r = 0.977), which means that both ELISA tests are suitable for epidemiological studies of A. marginale

Keywords
Anaplasma marginale - Babesia bovis - Babesia bigemina - ELISA - prevalence - semi-arid - Brazil

 
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